In Season 3 finale, Jane allots different room numbers to the suspects....The lady assassin first enters Betram's room and then Jane team moves on and she falls from the floors. 

I doubt that both Betram and O'Laughlin are associates of RJ. 
RJ was confused that both his accomplices gave different room nos that is why he sends his assassin to Betram's room first. After the "Rope" incident, Jane's suspicion moves on to O'laughlin alone.

If Betram is not an accomplice how would RJ knows that room no 605 is unoccupied. how can the assassin just like that go in to the room ? 


And why does Jane does not get hold of Bret Stiles ? He himself said he knows RJ a lot 

Pardon me for bad English